Journal: PLoS ONE

Article Title: Demineralized bone matrix used for direct pulp capping in rats

doi: 10.1371/journal.pone.0172693

Figure Lengend Snippet: (A): Representative immunohistochemical images of the blank control group, the Ca(OH) 2 group and the DBM group. a: The blank control group. Pulp morphology was normal. b- f: Ca(OH) 2 group (1, 3, 7, 14, 28 days). g- k: DBM group (1, 3, 7, 14, 28 days). (B): Mean IOD value of Runx2. *means significant difference.

Article Snippet: The sections were deparaffinized with xylene, hydrated in a series of descending grades of ethanol, and then rinsed briefly with PBS for the primary antibodies; the sections were incubated overnight at 4°C with polyclonal anti- Runx2, COL I, OCN and DSP (Wuhan Boster Biological Technology, Wuhan, China).

Techniques: Immunohistochemical staining, Control

Journal: Cell reports

Article Title: Nuclear Import Receptors Directly Bind to Arginine-Rich Dipeptide Repeat Proteins and Suppress Their Pathological Interactions

doi: 10.1016/j.celrep.2020.108538

Figure Lengend Snippet:

Article Snippet: Mouse anti TDP-43 , Proteintech , Cat# 60019-2-Ig.

Techniques: Recombinant, Protease Inhibitor, Staining, Virus, Plasmid Preparation, Software

Journal: Molecular Medicine Reports

Article Title: Downregulation of transgelin 2 promotes breast cancer metastasis by activating the reactive oxygen species/nuclear factor-κB signaling pathway

doi: 10.3892/mmr.2019.10643

Figure Lengend Snippet: Loss of TAGLN2 expression induces NF-κB activation. (A) Luciferase activity of an NF-κB-luciferase reporter plasmid in TAGLN2-overexpressing 293T cells was measured 48 h after stimulation with siRNA-TAGLN2 or scramble siRNA; luciferase activity was normalized to that of Renilla . Data were representative of three independent experiments. (B) Immunoblotting of IκB, p50, p65/RelA and TAGLN2 expression in MDA-MB-231 and MCF-7 cells transfected with pLKO.1-shRNA-TAGLN2 and pLKO.1-shRNA-scramble. The results of western blotting were representative of three independent experiments. (C) Cell migration was promoted in MDA-MB-231 cells transfected with pLKO.1-shRNA-TAGLN2 compared with cells treated with scramble shRNA. Treatment with 18 µM SN50 for 24 h reversed the effects of TAGLN2 downregulation on cell invasion. Data are representative of three independent experiments. *P<0.05 vs. scramble or as indicated. DMSO, dimethyl sulfoxide; NF-κB, nuclear factor-κB; shRNA, short hairpin RNA; siRNA, small interfering RNA; TAGLN2, transgelin 2.

Article Snippet: The membranes were probed with primary antibodies against PRDX1 (1:1,000; cat. no. EPR5433; Epitomics, Abcam, Cambridge, UK), FLAG (1:500; cat. no. F7425; Sigma-Aldrich, Merck KGaA), TAGLN2 (1:1,000; cat. no. 60044-1-lg; ProteinTech Group, Inc.), IκB (1:3,000; cat. no. 66418-1-lg; ProteinTech Group, Inc.), p50 (1:200; cat. no. 15506-1-AP; ProteinTech Group, Inc.), p65 (1:1,000; cat. no 10745-1-AP; ProteinTech Group, Inc.), MMP1 (1:1,000; cat. no. 54376; Cell Signaling Technology), MMP2 (1:1,000; cat. no. 4022; Cell Signaling Technology), CXCR4 (1:1,000; cat. no. ab181020; Abcam, Cambridge, UK), Lamin B1 (1:3,000; cat. no. 12987-1-AP; ProteinTech Group, Inc.) and GAPDH (1:3,000; cat. no. 60004-1-lg; ProteinTech Group, Inc.) on the rocking table at 4°C overnight.

Techniques: Expressing, Activation Assay, Luciferase, Activity Assay, Plasmid Preparation, Western Blot, Transfection, shRNA, Migration, Small Interfering RNA